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SuperArray Bioscience Corporation reaction readytm first-strand cdna synthesis kit
Reaction Readytm First Strand Cdna Synthesis Kit, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/readytm+cdna+kit/reactionready+first+strand+cdna+synthesis+kit/pm28973719-54-13-19
Average 90 stars, based on 1 article reviews
reaction readytm first-strand cdna synthesis kit - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Reverse Transcription:

Article Title: Genistein and daidzein act on a panel of genes implicated in cell cycle and angiogenesis by polymerase chain reaction arrays in human prostate cancer cell lines.
Article Snippet: Background: The prostate cancer most frequently affects men.. The ethnic origin and family antecedents of prostate cancer are established as risk factors.. The genetic factors associated with environmental factors such as the nutrition also play a role in the development of the cancer.

Article Title: Prolactin stimulates the proliferation of normal female cholangiocytes by differential regulation of Ca2+-dependent PKC isoforms.
Article Snippet: We used the RT2 Real-Time assay from SuperArray (Frederick, MD) to evaluate the expression of the short and long form of prolactin receptor mRNA in female cholangiocytes from normal and BDL rats. .. RNA was reverse transcribed using the Reaction ReadyTM First Strand cDNA synthesis kit (SuperArray). .. As described previously [55], 1 μl of the cDNA template was added to 12.5 μl of master mix, 10.5 μl of DI water and 1 μl of RT2 PCR rat primers (SuperArray, Frederick, MD) designed specifically for the messages for short and long prolactin receptor (Integrated DNA Technologies, Coralville, IA) and GAPDH (SuperArray).

Article Title: Transcriptional Activation of Endothelial Cells by TGFβ Coincides with Acute Microvascular Plasticity following Focal Spinal Cord Ischaemia/Reperfusion Injury
Article Snippet: .. Isolated RNA was reverse-transcribed using the Reaction ReadyTM First Strand cDNA synthesis Kit (SuperArray Bioscience). .. Differential gene expression was conducted using the RT2ProfilerTM PCR Array (rat, TGFb signalling PCR array; catalogue number PARN-035, SuperArray Bioscience). qRTPCR (quantitative real-time-PCR) was performed using an ABI 7900 real-time PCR instrument.

cDNA Synthesis:

Article Title: Genistein and daidzein act on a panel of genes implicated in cell cycle and angiogenesis by polymerase chain reaction arrays in human prostate cancer cell lines.
Article Snippet: Background: The prostate cancer most frequently affects men.. The ethnic origin and family antecedents of prostate cancer are established as risk factors.. The genetic factors associated with environmental factors such as the nutrition also play a role in the development of the cancer.

Article Title: Toll-Like Receptor 2 Is Required for Opioids-Induced Neuronal Apoptosis
Article Snippet: .. Briefly, first-strand cDNA was synthesized from 1μg of total RNA using a Reaction Ready first strand cDNA synthesis kit (SuperArray Bioscience Corporation). .. After incubation at 70 °C for 3 min and cooling down to 37 °C for 10 min, RT cocktail was added to the annealing mixture and further incubated at 37 °C for 60 min. 2μl of 1:2 diluted cDNA was subjected to real-time quantitative PCR using Bio-Rad iCycler iQ Multicolor Real-Time PCR Detection System (Bio-Rad).

Article Title: Opposing actions of endocannabinoids on cholangiocarcinoma growth: recruitment of Fas and Fas ligand to lipid rafts.
Article Snippet: .. Reverse Transcription-PCR Analysis—We evaluated the expression of the cannabinoid receptors by reverse transcription-PCR using total RNA (1 g) from H69, Mz-ChA-1, HuH28, HUCC-T1, and SG231 cholangiocarcinoma cells. cDNA synthesis was performed using the Reaction ReadyTM first strand cDNA synthesis kit from SuperArray. ..

Article Title: Prolactin stimulates the proliferation of normal female cholangiocytes by differential regulation of Ca2+-dependent PKC isoforms.
Article Snippet: We used the RT2 Real-Time assay from SuperArray (Frederick, MD) to evaluate the expression of the short and long form of prolactin receptor mRNA in female cholangiocytes from normal and BDL rats. .. RNA was reverse transcribed using the Reaction ReadyTM First Strand cDNA synthesis kit (SuperArray). .. As described previously [55], 1 μl of the cDNA template was added to 12.5 μl of master mix, 10.5 μl of DI water and 1 μl of RT2 PCR rat primers (SuperArray, Frederick, MD) designed specifically for the messages for short and long prolactin receptor (Integrated DNA Technologies, Coralville, IA) and GAPDH (SuperArray).

Article Title: Toll-like receptor pathway signaling is differently regulated in neutrophils and peripheral mononuclear cells of patients with sepsis, severe sepsis, and septic shock*
Article Snippet: Monocytes and neutrophils play a central role in the pathophysiology of sepsis, and are involved in both the protective response and sequelae of the syndrome (1–3).. Cellular interaction with bacteria and their products occurs through the preserved structures in the microorganisms, such as lipopolysaccharide (LPS), lipoproteins, and peptidoglycan, collectively named pathogen-associated molecular pattern, that can be recognized by the pattern recognition receptors, among which, CD14 and Tolllike receptors (TLR) (4, 5).. Cellular response to LPS and to other microbial components is modulated during clinical sepsis, yet the mechanisms are only partially understood.

Article Title: Transcriptional Activation of Endothelial Cells by TGFβ Coincides with Acute Microvascular Plasticity following Focal Spinal Cord Ischaemia/Reperfusion Injury
Article Snippet: .. Isolated RNA was reverse-transcribed using the Reaction ReadyTM First Strand cDNA synthesis Kit (SuperArray Bioscience). .. Differential gene expression was conducted using the RT2ProfilerTM PCR Array (rat, TGFb signalling PCR array; catalogue number PARN-035, SuperArray Bioscience). qRTPCR (quantitative real-time-PCR) was performed using an ABI 7900 real-time PCR instrument.

other:

Article Title: H3 histamine receptor agonist inhibits biliary growth of BDL rats by downregulation of the cAMP-dependent PKA/ERK1/2/ELK-1 pathway
Article Snippet: Following immunohistochemistry or immunofluorescence, samples were observed either with the light microscope ECLIPSE E600 (Nikon, Kawasaki, Japan) or fluorescence microscope DMRXA/HC (LEICA, Tokyo, Japan).

Synthesized:

Article Title: Toll-Like Receptor 2 Is Required for Opioids-Induced Neuronal Apoptosis
Article Snippet: .. Briefly, first-strand cDNA was synthesized from 1μg of total RNA using a Reaction Ready first strand cDNA synthesis kit (SuperArray Bioscience Corporation). .. After incubation at 70 °C for 3 min and cooling down to 37 °C for 10 min, RT cocktail was added to the annealing mixture and further incubated at 37 °C for 60 min. 2μl of 1:2 diluted cDNA was subjected to real-time quantitative PCR using Bio-Rad iCycler iQ Multicolor Real-Time PCR Detection System (Bio-Rad).

Expressing:

Article Title: Opposing actions of endocannabinoids on cholangiocarcinoma growth: recruitment of Fas and Fas ligand to lipid rafts.
Article Snippet: .. Reverse Transcription-PCR Analysis—We evaluated the expression of the cannabinoid receptors by reverse transcription-PCR using total RNA (1 g) from H69, Mz-ChA-1, HuH28, HUCC-T1, and SG231 cholangiocarcinoma cells. cDNA synthesis was performed using the Reaction ReadyTM first strand cDNA synthesis kit from SuperArray. ..

Isolation:

Article Title: Transcriptional Activation of Endothelial Cells by TGFβ Coincides with Acute Microvascular Plasticity following Focal Spinal Cord Ischaemia/Reperfusion Injury
Article Snippet: .. Isolated RNA was reverse-transcribed using the Reaction ReadyTM First Strand cDNA synthesis Kit (SuperArray Bioscience). .. Differential gene expression was conducted using the RT2ProfilerTM PCR Array (rat, TGFb signalling PCR array; catalogue number PARN-035, SuperArray Bioscience). qRTPCR (quantitative real-time-PCR) was performed using an ABI 7900 real-time PCR instrument.



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